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Gating strategy and frequencies of CD4 + , CD8 + , and CD19 + cells. In the first step, the FSC/SSC blot was used to differentiate between living cells and cell debris. Next, gating was performed on the basis of the CD8/CD4 blot to dissect CD8 + and CD4 + cells, and on the basis of the CD19/SSC blot to dissect CD19 + cells. Pseudocolor plots are used to visualize the density of cell population(s). Representative dot plots illustrating the gating strategy are shown, as well as the frequencies of CD4 + , CD8 + , CD19 + cells for the <t>baricitinib-treated</t> and the vehicle-treated group.
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Gating strategy and frequencies of CD4 + , CD8 + , and CD19 + cells. In the first step, the FSC/SSC blot was used to differentiate between living cells and cell debris. Next, gating was performed on the basis of the CD8/CD4 blot to dissect CD8 + and CD4 + cells, and on the basis of the CD19/SSC blot to dissect CD19 + cells. Pseudocolor plots are used to visualize the density of cell population(s). Representative dot plots illustrating the gating strategy are shown, as well as the frequencies of CD4 + , CD8 + , CD19 + cells for the <t>baricitinib-treated</t> and the vehicle-treated group.
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Gating strategy and frequencies of CD4 + , CD8 + , and CD19 + cells. In the first step, the FSC/SSC blot was used to differentiate between living cells and cell debris. Next, gating was performed on the basis of the CD8/CD4 blot to dissect CD8 + and CD4 + cells, and on the basis of the CD19/SSC blot to dissect CD19 + cells. Pseudocolor plots are used to visualize the density of cell population(s). Representative dot plots illustrating the gating strategy are shown, as well as the frequencies of CD4 + , CD8 + , CD19 + cells for the <t>baricitinib-treated</t> and the vehicle-treated group.
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Gating strategy and frequencies of CD4 + , CD8 + , and CD19 + cells. In the first step, the FSC/SSC blot was used to differentiate between living cells and cell debris. Next, gating was performed on the basis of the CD8/CD4 blot to dissect CD8 + and CD4 + cells, and on the basis of the CD19/SSC blot to dissect CD19 + cells. Pseudocolor plots are used to visualize the density of cell population(s). Representative dot plots illustrating the gating strategy are shown, as well as the frequencies of CD4 + , CD8 + , CD19 + cells for the baricitinib-treated and the vehicle-treated group.

Journal: International Journal of Molecular Sciences

Article Title: Effect of the JAK Inhibitor Baricitinib on Cytokine Production and Bone Properties in a Mouse Model of Accelerated Aging

doi: 10.3390/ijms27115047

Figure Lengend Snippet: Gating strategy and frequencies of CD4 + , CD8 + , and CD19 + cells. In the first step, the FSC/SSC blot was used to differentiate between living cells and cell debris. Next, gating was performed on the basis of the CD8/CD4 blot to dissect CD8 + and CD4 + cells, and on the basis of the CD19/SSC blot to dissect CD19 + cells. Pseudocolor plots are used to visualize the density of cell population(s). Representative dot plots illustrating the gating strategy are shown, as well as the frequencies of CD4 + , CD8 + , CD19 + cells for the baricitinib-treated and the vehicle-treated group.

Article Snippet: A total of 27 mice were treated twice-daily with 10 mg/kg body weight baricitinib (6.73 mM; MedChemExpress; 1187594-09-7, Monmouth Junction, NJ, USA) dissolved in 0.5% methylcellulose and 0.025% Tween 20 solution, and 30 mice received a vehicle (0.5% methylcellulose and 0.025% Tween 20 solution).

Techniques:

Bone turnover markers. Scatter dot plots show serum levels of PINP and CTX in baricitinib-treated ( n = 26) and vehicle-treated ( n = 29) SAMP8 mice. All data are not normally distributed; the Mann–Whitney U test was used. Horizontal lines indicate the median.

Journal: International Journal of Molecular Sciences

Article Title: Effect of the JAK Inhibitor Baricitinib on Cytokine Production and Bone Properties in a Mouse Model of Accelerated Aging

doi: 10.3390/ijms27115047

Figure Lengend Snippet: Bone turnover markers. Scatter dot plots show serum levels of PINP and CTX in baricitinib-treated ( n = 26) and vehicle-treated ( n = 29) SAMP8 mice. All data are not normally distributed; the Mann–Whitney U test was used. Horizontal lines indicate the median.

Article Snippet: A total of 27 mice were treated twice-daily with 10 mg/kg body weight baricitinib (6.73 mM; MedChemExpress; 1187594-09-7, Monmouth Junction, NJ, USA) dissolved in 0.5% methylcellulose and 0.025% Tween 20 solution, and 30 mice received a vehicle (0.5% methylcellulose and 0.025% Tween 20 solution).

Techniques: MANN-WHITNEY

µCT analysis of the femur and the fourth vertebral body. Parameters derived from the femur are shown for baricitinib-treated ( n = 26) and vehicle-treated ( n = 29) SAMP8 mice. ( A ) Cortical parameters: average cortical thickness (Ct.Th), cortical bone area fraction (Ct.Ar/Tt.Ar), cortical bone area (Ct.Ar), total cross-sectional area (Tt.Ar). ( B ) Trabecular parameters: bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp). ( C ) Parameters of the lumbar spine are presented as follows: bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp). Data are not normally distributed; Mann–Whitney was used except for Ct.Ar and BV/TV in the femur and BV/TV, Tb.Th and Tb.Sp in the vertebral body; here, unpaired t -tests were used. Horizontal lines indicate the median.

Journal: International Journal of Molecular Sciences

Article Title: Effect of the JAK Inhibitor Baricitinib on Cytokine Production and Bone Properties in a Mouse Model of Accelerated Aging

doi: 10.3390/ijms27115047

Figure Lengend Snippet: µCT analysis of the femur and the fourth vertebral body. Parameters derived from the femur are shown for baricitinib-treated ( n = 26) and vehicle-treated ( n = 29) SAMP8 mice. ( A ) Cortical parameters: average cortical thickness (Ct.Th), cortical bone area fraction (Ct.Ar/Tt.Ar), cortical bone area (Ct.Ar), total cross-sectional area (Tt.Ar). ( B ) Trabecular parameters: bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp). ( C ) Parameters of the lumbar spine are presented as follows: bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp). Data are not normally distributed; Mann–Whitney was used except for Ct.Ar and BV/TV in the femur and BV/TV, Tb.Th and Tb.Sp in the vertebral body; here, unpaired t -tests were used. Horizontal lines indicate the median.

Article Snippet: A total of 27 mice were treated twice-daily with 10 mg/kg body weight baricitinib (6.73 mM; MedChemExpress; 1187594-09-7, Monmouth Junction, NJ, USA) dissolved in 0.5% methylcellulose and 0.025% Tween 20 solution, and 30 mice received a vehicle (0.5% methylcellulose and 0.025% Tween 20 solution).

Techniques: Derivative Assay, MANN-WHITNEY

Bone histomorphometry of the fifth vertebral body. Trabecular and static bone histomorphometric parameters are shown: ( A ) bone volume/total volume (BV/TV) p = 0.024, trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp); ( B ) number of osteoblasts/bone perimeter (N.Ob/B.Pm), number of osteoclasts/bone perimeter (N.Oc/B.Pm), number of osteocytes/bone area (N.Ot/B.Ar), and eroded surface/bone surface (ES/BS). Group comparison of baricitinib-treated ( n = 26) and vehicle-treated ( n = 29) SAMP8 mice. Each dot represents one animal. * p < 0.05. Data are not normally distributed; Mann–Whitney U was used except for Tb.Th, Tb.Sp and N.Ot/B.Ar; here, unpaired t -tests were used. Mean values are used for normally distributed and median values for non-normally distributed data. Horizontal lines indicate the median.

Journal: International Journal of Molecular Sciences

Article Title: Effect of the JAK Inhibitor Baricitinib on Cytokine Production and Bone Properties in a Mouse Model of Accelerated Aging

doi: 10.3390/ijms27115047

Figure Lengend Snippet: Bone histomorphometry of the fifth vertebral body. Trabecular and static bone histomorphometric parameters are shown: ( A ) bone volume/total volume (BV/TV) p = 0.024, trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp); ( B ) number of osteoblasts/bone perimeter (N.Ob/B.Pm), number of osteoclasts/bone perimeter (N.Oc/B.Pm), number of osteocytes/bone area (N.Ot/B.Ar), and eroded surface/bone surface (ES/BS). Group comparison of baricitinib-treated ( n = 26) and vehicle-treated ( n = 29) SAMP8 mice. Each dot represents one animal. * p < 0.05. Data are not normally distributed; Mann–Whitney U was used except for Tb.Th, Tb.Sp and N.Ot/B.Ar; here, unpaired t -tests were used. Mean values are used for normally distributed and median values for non-normally distributed data. Horizontal lines indicate the median.

Article Snippet: A total of 27 mice were treated twice-daily with 10 mg/kg body weight baricitinib (6.73 mM; MedChemExpress; 1187594-09-7, Monmouth Junction, NJ, USA) dissolved in 0.5% methylcellulose and 0.025% Tween 20 solution, and 30 mice received a vehicle (0.5% methylcellulose and 0.025% Tween 20 solution).

Techniques: Comparison, MANN-WHITNEY